While negative effects of landscape fragmentation on biodiversity are well documented, recent evidence suggests positive or neutral effects may also be common. The mechanisms driving these contrasting outcomes cannot be understood without assessing how species traits influence responses to landscape and patch characteristics. Here, we show that three key elements can enhance predictability of fragmentation effects across scales: (i) the trait distribution of the regional species pool; (ii) the relationship between taxonomic and trait diversity; and (iii) the effects of the landscape matrix on the distribution of species traits. Considering these elements will facilitate the development of generalizable hypotheses on the consequences of fragmentation across diverse taxonomic groups and regions, with broad applicability to ecology and conservation.